CST, 32621S, Human CD40 Ligand (hCD40L) Recombinant Protein

Growth Factors and Cytokines for studying CD40LG in the research area. Product Usage Information Working concentration of recombinant hCD40L generally ranges from 1-100 ng/ml. Storage Recombinant human CD40L is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC. Source / Purification Recombinant human CD40L was expressed in and is supplied in a lyophilized form. Background CD40 Ligand (CD40L), a member of the TNF superfamily of ligands, is expressed as either a membrane-bound or soluble homotrimer (1). Both membrane-bound and soluble forms of CD40L have biological activity (1,2). CD40L is expressed primarily on activated T cells; however, mast cells, basophils, and NK cells may also express CD40L (1). CD40L functions as an important T cell co-stimulatory molecule and enhances T-dependent B cell responses (1,2). CD40L binds CD40, which is a receptor expressed on B cells, dendritic cells, and macrophages (1). Binding of CD40L to CD40 leads to the recruitment of TRAF proteins (TRAFs 2,3,5, and 6) resulting in the activation of the JNK and NF-κB pathways (1,2). Alternate Names CD154; CD40LG; gp39; T-BAM; TNFSF5; TRAP