CST, 3262S, BLK Antibody

Polyclonal Antibody for studying BLK. Validated for Western Blotting. Highly specific and rigorously validated in-house, BLK Antibody (CST #3262) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BLK Antibody detects endogenous levels of total BLK. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy-terminus of human BLK. Antibodies are purified by protein A and peptide affinity chromatography. Background BLK is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of BLK (3). It has been suggested that BLK is involved in the control of B cell differentiation and proliferation (4,5). BLK transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that BLK may play an important role in thymopoiesis (6). BLK function may be redundant, however, as mice that do not express BLK are not impaired with respect to B cell development and immune response (7). Alternate Names B lymphocyte kinase; B lymphoid tyrosine kinase; BLK; BLK nonreceptor tyrosine kinase; BLK proto-oncogene, Src family tyrosine kinase; MGC10442; MODY11; p55-Blk; Tyrosine-protein kinase Blk Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit