CST, 3264S, Ero1-L alpha Antibody

Polyclonal Antibody for studying Ero1L. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Ero1-L alpha Antibody (CST #3264) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ero1-L alpha Antibody detects endogenous levels of total Ero1-Lα protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu218 of human Ero1-Lα. Antibodies are purified by protein A and peptide affinity chromatography. Background Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Several oxidoreductases of the protein disulfide isomerase (PDI) family essential for disulfide formation and isomerization are localized to the ER (2). Studies have found that the ER-residing protein endoplasmic oxidoreductin-1 (Ero1) provides the oxidizing potential to the ER in (3). experiments demonstrated that Ero1 is oxidized by molecular oxygen in a FAD-dependent manner and the oxidized Ero1 in turn serves as an oxidant for PDI (4). Two human homologs of Ero1, Ero1-like (Ero1-Lα and β) have been identified (2,5). Ero1-Lα is an ER membrane-associated N-glycoprotein that promotes oxidative protein folding and has been shown to be expressed in several cell lines and tissues (2). Alternate Names Endoplasmic oxidoreductin-1-like protein; endoplasmic reticulum oxidoreductase 1 alpha; Endoplasmic reticulum oxidoreductase alpha; ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like protein alpha; ERO1A; Ero1alpha; ERO1L; ERO1LA; Oxidoreductin-1-L-alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit