CST, 3270T, Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JUN (Ser73) phosphate. Validated for WB,IP,IHC,IF,IF,F,ChIP,ChIP,C&R,C&T. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody (CST #3270) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Frozen): 1:800 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser73. This antibody may also recognize JunD phosphorylated at Ser100. Species Reactivity: Human, Mouse, Rat, Monkey, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun. Background c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals, including growth factors, chemokines, and stress, activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knockout studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes, including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions, including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12). Alternate Names Activator protein 1; AP-1; AP1; c-Jun; cJUN; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; p39; Proto-oncogene c-Jun; proto-oncogene cJun; Transcription factor AP-1; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog Specification REACTIVITY: H M R Mk Pg SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG