CST, 3274S, G-beta-L (86B8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GbetaL. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, G-beta-L (86B8) Rabbit Monoclonal Antibody (CST #3274) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity G-beta-L (86B8) Rabbit Monoclonal Antibody detects endogenous levels of total GβL protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification GβL (86B8) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gln210 of human GβL. Background Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease phosphorylation of S6K1 (2). Alternate Names G protein beta subunit like; G protein beta subunit-like; Gable; GbetaL; GBL; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; MLST8; MTOR associated protein, LST8 homolog; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG