CST, 3279S, Shootin1 Antibody

Polyclonal Antibody for studying Shootin1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Shootin1 Antibody (CST #3279) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Shootin1 Antibody detects endogenous levels of total shootin1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Lys450 of human shootin1. Antibodies are purified by peptide affinity chromatography. Background The differentiation process of neurons can be divided into five stages, each stage characterized by morphological changes observed in the developing cells. In stage 1, the cells extend lamellipodia and in stage 2 their lamellipodia develop into immature neurites. In stage 3 one neurite elongates rapidly to form an axon and in stage 4 the remaining immature neuritis elongate to form dendrites. In stage 5 synaptic contacts are formed and a neuronal network is established (1). Shootin1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. The extension of an axon requires considerable reorganization of the cytoskeleton mediated by PI3K/Akt and PI3K/Cdc42 signaling (1). Shootin1 is involved in regulating the subcellular localization of PI3 kinase. Furthermore, shootin1 is upregulated during polarization and accumulates asymmetrically in a single neurite that consequently elongates rapidly to form an axon (2). Alternate Names DKFZp686A0439; KIAA1598; MGC40476; shootin 1; Shootin-1; SHOOTIN1; SHOT1; SHTN1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit