CST, 32823S, SPON2 Antibody

Polyclonal Antibody for studying SPON2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SPON2 Antibody (CST #32823) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SPON2 Antibody recognizes endogenous levels of total SPON2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu273 of human SPON2 protein. Antibodies are purified by peptide affinity chromatography. Background SPON2 (spondin 2, mindin) is an extracellular matrix protein belonging to the mindin/F-spondin family (1). The protein has two amino terminal follistatin-like (FS) domains and a carboxy terminal thrombospondin type 1 repeat (TSR) domain. The FS domains mediate interactions between SPON2 and integrins, whereas the TSR domain contributes to the role of SPON2 as a pathogen pattern recognition molecule (2). In that capacity, SPON2 plays an important role in initiation of the innate immune response. It functions as a pattern recognition molecule within the extracellular matrix, where it binds to microbial pathogens and presents them to immune cells (3). Interactions between SPON2 and integrins are also critical for inflammatory cell recruitment and T cell priming (4,5). In addition to its role in innate immunity, SPON2 has also been shown to play a role in hippocampal neuronal outgrowth, cancer metastasis, and diabetic nephropathy (6-8). Alternate Names Differentially expressed in cancerous and non-cancerous lung cells 1; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ22401; FLJ34460; M-spondin; Mindin; SPON2; spondin 2; spondin 2, extracellular matrix protein; Spondin-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit