CST, 32850S, SIGIRR (E5M2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIGIRR. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SIGIRR (E5M2V) Rabbit Monoclonal Antibody (CST #32850) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SIGIRR (E5M2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIGIRR protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly391 of human SIGIRR protein. Background SIGIRR (Single Ig IL-1-related receptor), also known as IL-1R8, belongs to the IL-1 receptor family characterized by the presence of a common intracellular Toll-IL-1 resistance (TIR) domain and extracellular Ig-like domains (1-3). SIGIRR is widely expressed in several epithelial tissues such as liver, lung, and kidney, and in leukocytes including monocytes, T and B lymphocytes, dendritic cells, and natural killer (NK) cells. It negatively modulates the activation upon ligand binding of certain TIR-containing receptors including members of IL-1 receptor family and TLR family (4-6). Recently SIGIRR was identified as a crucial checkpoint receptor in NK cells and a potential therapeutic target in several pathological conditions (7,8). Alternate Names IL-1R8; MGC110992; SIGIR; SIGIRR; single Ig and TIR domain containing; Single Ig IL-1-related receptor; Single Ig IL-1R-related molecule; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; single immunoglobulin domain IL1R1 related; Single immunoglobulin domain-containing IL1R-related protein; TIR8; Toll/interleukin-1 receptor 8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG