CST, 32920S, UBE3C (F2S2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBE3C. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, UBE3C (F2S2J) Rabbit Monoclonal Antibody (CST #32920) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UBE3C (F2S2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBE3C protein. Reactivity for immunofluorescence is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly660 of human UBE3C protein. Background Ubiquitin-Protein Ligase E3C (UBE3C) is an E3 ubiquitin-protein ligase that contributes to the regulation of multiple cellular processes through ubiquitination of various protein substrates, targeting them for proteasomal degradation. UBE3C activates the Wnt/β-catenin pathway, promoting proliferation and inhibiting apoptosis in cancer cells (1,2). UBE3C-mediated protein degradation also regulates autophagy signaling (3,4), viral replication (5), type I interferon signaling (6), and radioresistance (7). In addition, UBE3C participates in eliminating misfolded protein aggregates by targeting misfolded proteins for degradation (8). Alternate Names HECT-type ubiquitin transferase E3C; HectH2; KIAA0010; KIAA10; UBE3C; ubiquitin protein ligase E3C; ubiquitin-protein isopeptide ligase (E3); Ubiquitin-protein ligase E3C Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 123 Source/Isotype: Rabbit IgG