CST, 3299S, Annexin A1 Antibody

Polyclonal Antibody for studying ANXA1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Annexin A1 Antibody (CST #3299) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Annexin A1 Antibody detects endogenous levels of total annexin A1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human annexin A1. Antibodies are purified by peptide affinity chromatography. Background The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14). Alternate Names Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; ANX1; anxa 1; ANXA1; Calpactin II; Calpactin-2; Chromobindin-9; epididymis secretory sperm binding protein; Lipocortin I; LPC1; p35; Phospholipase A2 inhibitory protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 37 SOURCE: Rabbit