CST, 3302S, GCN2 Antibody

Polyclonal Antibody for studying GCN2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GCN2 Antibody (CST #3302) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GCN2 Antibody detects endogenous levels of GCN2 protein independent of phosphorylation. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequence near the amino terminus of human GCN2. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), and hemin deficiency (HRI) can phosphorylate the eIF2 alpha subunit (1,2). GCN2 is also required for UV light-induced translation inhibition, and phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-κB also requires GCN2, which may act simply by preventing translation of IκB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6). Alternate Names E2AK4; eIF-2-alpha kinase GCN2; EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; KIAA1338; PVOD2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 220 SOURCE: Rabbit