CST, 3303S, HSPA4/Apg-2 Antibody

Polyclonal Antibody for studying HSPA4. Validated for Western Blotting. Highly specific and rigorously validated in-house, HSPA4/Apg-2 Antibody (CST #3303) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HSPA4/Apg-2 Antibody detects endogenous levels of total HSPA4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPA4. Antibodies are purified by protein A and peptide affinity chromatography. Background The heat shock protein HSPA4 (Apg-2, HSP70RY) is a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins (1). Apg-2 has chaperone ability similiar to Hsp110, and it plays a role under non-stress conditions (2). Apg-2 interacts with TJP1/ZO-1 and functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress (3,4). Alternate Names APG-2; APG2; epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 4; heat shock 70kDa protein 4; heat shock protein family A (Hsp70) member 4; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; hsp70; hsp70 RY; HSP70RY; HSP74; HSPA4; HSPH2; MGC131852; RY Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit