CST, 33056S, b-Myb (F9W2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying B-Myb/MYBL2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, b-Myb (F9W2M) Rabbit Monoclonal Antibody (CST #33056) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity b-Myb (F9W2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total b-Myb protein. This antibody may detect a nonspecific band at 60 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human b-Myb protein. Background b-Myb, also known as MYBL2, is a member of the MYB transcription factor family and plays key regulatory roles in cell cycle, proliferation, and differentiation. b-Myb is essentially expressed in all proliferating cells, and b-Myb knockout mice are embryonically lethal (1,2). Importantly, b-Myb is overexpressed and reported to play a critical role in driving several cancers, including breast cancer, hepatocellular carcinoma, renal cell carcinoma, and non-small cell lung cancer (3-7). Alternate Names B-Myb; BMYB; MGC15600; MYB proto-oncogene like 2; Myb-like protein 2; Myb-related protein B; MYBB; MYBL2; v-myb avian myeloblastosis viral oncogene homolog-like 2; v-myb myeloblastosis viral oncogene homolog (avian)-like 2; v-myb myeloblastosis viral oncogene homolog-like 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG