CST, 3307T, VRK1 (1F6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying VRK1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, VRK1 (1F6) Mouse Monoclonal Antibody (CST #3307) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VRK1 (1F6) Mouse Monoclonal Antibody detects endogenous levels of total VRK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant fusion protein GST-full length human VRK1. Background The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses Erk activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation. Alternate Names MGC117401; MGC138280; MGC142070; PCH1; PCH1A; Serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1; VRK serine/threonine kinase 1; VRK1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Mouse IgG1