CST, 33121S, MICAL2 (F7N1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MICAL2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MICAL2 (F7N1B) Rabbit Monoclonal Antibody (CST #33121) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MICAL2 (F7N1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MICAL2 protein. Based on the sequence of the protein antigen, this antibody is expected to detect all isoforms of MICAL2. This antibody detects an approximately 27 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the central region of human MICAL2 protein. This antibody recognizes residues surrounding Pro1093 of human MICAL2 protein. Background Molecule interacting with CasL protein 2 (MICAL2) is a flavin protein monooxygenase that regulates multiple cellular processes, including proliferation, migration, cell transport, and apoptosis, by promoting the depolymerization of F-actin. MICAL2 regulates serum response factor (SRF)/myocardin-related transcription factor-A (MRTF-A)-mediated transcription by modulating nuclear G-actin levels (1). MICAL2 is overexpressed in human cancer and promotes tumor progression and metastasis by inducing epithelial-mesenchymal transition (EMT) (2). In human pancreatic ductal adenocarcinoma (PDAC), was identified as a possible super-enhancer (SE)-associated gene whose overexpression promotes KRAS signaling, proliferation, and migration (3). Alternate Names [F-actin]-methionine sulfoxide oxidase MICAL2; [F-actin]-monooxygenase MICAL2; DKFZp686H03148; DKFZp686H2469; flavoprotein oxidoreductase MICAL2; KIAA0750; MICA2; MICAL-2; MICAL2; MICAL2PV1; MICAL2PV2; MICALCL; microtubule associated monooxygenase, calponin and LIM domain containing 2; microtubule associated monoxygenase, calponin and LIM domain containing 2; Molecule interacting with CasL protein 2; Protein MICAL-2; protein-methionine sulfoxide oxidase MICAL2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 127 Source/Isotype: Rabbit IgG