CST, 3313L, Phospho-Cofilin (Ser3) (77G2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cofilin 1 (Ser3) phosphate. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Cofilin (Ser3) (77G2) Rabbit Monoclonal Antibody (CST #3313) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 75735 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Cofilin (Ser3) (77G2) Rabbit Monoclonal Antibody detects endogenous levels of cofilin only when phosphorylated at serine 3. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Background Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). Alternate Names 18 kDa phosphoprotein; CFL; CFL1; COF1; cofilin; cofilin 1; cofilin 1 (non-muscle); Cofilin-1; Cofilin, non-muscle isoform; epididymis secretory protein Li 15; HEL-S-15; p18 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG