CST, 3314S, JMJD1B (C69G2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JMJD1B. Validated for WB,IP,IF,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, JMJD1B (C69G2) Rabbit Monoclonal Antibody (CST #3314) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:25 Immunofluorescence (Immunocytochemistry): 1:200 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), CUT&RUN Specificity / Sensitivity JMJD1B (C69G2) Rabbit Monoclonal Antibody detects endogenous levels of JMJD1B protein (all three isoforms). This antibody does not cross react with other Jumonji C proteins, including HR, JMJD1A and JMJD1C. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr579 of the human JMJD1B protein. Background The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8). Alternate Names [histone H3]-dimethyl-L-lysine(9) demethylase 3B; 5qNCA; C5orf7; DIJOS; JHDM2B; JmjC domain-containing histone demethylation protein 2B; JMJD1B; jumonji domain containing 1B; Jumonji domain-containing protein 1B; KDM3B; KIAA1082; lysine (K)-specific demethylase 3B; lysine demethylase 3B; Lysine-specific demethylase 3B; NET22; Nuclear protein 5qNCA Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG