CST, 33156T, PML (E5R8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PML human. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PML (E5R8T) Rabbit Monoclonal Antibody (CST #33156) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PML (E5R8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PML protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro364 of human PML protein. Background Promyelocytic leukemia protein (PML) functions via its association with PML bodies in the nucleus (1). PML bodies are dynamic protein aggregates that are interspersed between chromatin in the nuclei of most mammalian cells. The PML protein acts as a scaffold in PML bodies to recruit other proteins, a process regulated by post-translational modifications, such as sumoylation. PML bodies function to regulate a large number of cellular processes, such as tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense (1). The chromosomal translocation t(15;17)(q21;q21) involving the fusion of PML and RARalpha is found in acute promyelocytic leukemia (APL), while a second translocation t(9;15)(p13;q24) involving the fusion of PML and PAX5 was found in acute lymphoblastic leukemia (ALL) (2-6). In addition, PML is frequently inactivated or downregulated in cancer. Alternate Names MYL; PML; PML nuclear body scaffold; PML/RARA fusion; PP8675; Probable transcription factor PML; promyelocytic leukemia; promyelocytic leukemia protein; promyelocytic leukemia, inducer of; Protein PML; RING finger protein 71; RNF71; TRIM19; tripartite motif protein TRIM19; Tripartite motif-containing protein 19 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 48-200 Source/Isotype: Rabbit IgG