CST, 33176S, ZIP7/SLC39A7 (D1O3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC39A7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZIP7/SLC39A7 (D1O3A) Rabbit Monoclonal Antibody (CST #33176) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZIP7/SLC39A7 (D1O3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZIP7 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu201 of human ZIP7 protein. Background The solute carrier family 39 (zinc transporter) member 7 (SLC39A7, ZIP7) is an ER and Golgi membrane protein that regulates cellular zinc homeostasis by controlling release of zinc from these organelles to the cytoplasm (1,2). Zinc release mediated by ZIP7 results in activation of protein kinases that are involved in cell proliferation and migration (3,4). The protein kinase CK2 phosphorylates and activates ZIP7 in response to extracellular signals, such as growth factor stimulation (4,5). Increased expression of ZIP7 is observed in breast cancer tissues (6). Research studies indicate that ZIP7 is responsible for activation of multiple tyrosine kinases in aggressive, tamoxifen-resistant breast cancer (7,8). Alternate Names D6S115E; D6S2244E; H2-KE4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene, mouse, human homolog of; Really interesting new gene 5 protein; RING5; S39A7; SLC39A7; solute carrier family 39 (zinc transporter), member 7; Solute carrier family 39 member 7; Zinc transporter SLC39A7; ZIP7; Zrt-, Irt-like protein 7 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG