CST, 3318S, Cofilin (D59) Antibody

Polyclonal Antibody for studying cofilin 1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cofilin (D59) Antibody (CST #3318) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cofilin (D59) Antibody detects endogenous levels of total cofilin protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp59 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography. Â Background Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). Alternate Names 18 kDa phosphoprotein; CFL; CFL1; COF1; cofilin; cofilin 1; cofilin 1 (non-muscle); Cofilin-1; Cofilin, non-muscle isoform; epididymis secretory protein Li 15; HEL-S-15; p18 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 19 SOURCE: Rabbit