CST, 33210S, SAM68 (E6Y2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Sam68. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SAM68 (E6Y2F) Rabbit Monoclonal Antibody (CST #33210) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SAM68 (E6Y2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAM68 protein. This antibody does not cross-react with KHDRBS2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly402 of human SAM68 protein. Background Src associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8). Alternate Names FLJ34027; GAP-associated tyrosine phosphoprotein p62; GAP-associated tyrosine phosphoprotein p62 (Sam68); KH domain containing, RNA binding, signal transduction associated 1; KH domain-containing, RNA-binding, signal transduction-associated protein 1; KH RNA binding domain containing, signal transduction associated 1; KHDR1; KHDRBS1; p21 Ras GTPase-activating protein-associated p62; p62; p68; SAM68; Src-associated in mitosis 68 kDa protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG