CST, 3321S, Phospho-Ras-GRF1 (Ser916) Antibody

Polyclonal Antibody for studying RasGRF1 (Ser916) phosphate mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Ras-GRF1 (Ser916) Antibody (CST #3321) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Ras-GRF1 (Ser916) Antibody detects transfected levels of Ras-GRF1 only when phosphorylated at serine 916. This antibody does not cross-react with phosphorylated Ras-GRF2. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 916 of mouse Ras-GRF1. Antibodies are purified by protein A and peptide affinity chromatography. Background Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5). Alternate Names AI844718; Cdc25; CDC25Mm; GNRP; Grf; GRF beta; Grf1; Grfbe; Grfbeta; guanine nucleotide releasing factor 1; Guanine nucleotide-releasing protein; p190; P190-A; p190RhoGEF; Ras guanine release factor beta; RAS protein-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; Rasgrf1; RGRF1 Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 155 SOURCE: Rabbit