CST, 33254S, S100A8 (F4R2W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying S100A8. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, S100A8 (F4R2W) Rabbit Monoclonal Antibody (CST #33254) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1250 - 1:5000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:300 - 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37924 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity S100A8 (F4R2W) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100A8 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human S100A8 protein. Background S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9). Alternate Names 60B8AG; CAGA; calgranulin A; Calgranulin-A; Calprotectin L1L subunit; CFAG; CGLA; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; MRP8; NIF; p8; Protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100A8; S10A8; Urinary stone protein band A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 11 Source/Isotype: Rabbit IgG