CST, 3327S, Phospho-Dab1 (Tyr220) Antibody

Polyclonal Antibody for studying Dab1 (Tyr220) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Dab1 (Tyr220) Antibody (CST #3327) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Dab1 (Tyr220) Antibody detects transfected levels of Dab1 protein only when phosphorylated at tyrosine 220. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human Dab1. Antibodies are purified by protein A and peptide affinity chromatography. Background The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB domain contains a pleckstrin homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL, and Dock1 associate with tyrosine-phosphorylated Dab1. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4). Alternate Names DAB adaptor protein 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1; DAB1; DAB1, reelin adaptor protein; Disabled homolog 1; disabled homolog 1 (Drosophila); SCA37 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 80, 110 GFP-Dab1 fusion. SOURCE: Rabbit