CST, 33293S, CD11c (N418) Hamster Monoclonal Antibody (APC Conjugate)

Monoclonal Antibody for studying ITGAX mouse. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD11c (N418) Hamster Monoclonal Antibody (APC Conjugate) (CST #33293) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.125 μg of antibody per test. Flow Cytometry (Live): 1:160 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD11c (N418) Hamster Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD11c protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7). The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9). Alternate Names AI449405; CD11 antigen-like family member C; Cd11c; CD11C (p150) alpha polypeptide; complement receptor 4; Cr4; integrin alpha X; Integrin alpha-X; integrin aX; ITAX; Itgax; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; mFLJ00114; MFLJ00114 protein; N418 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Hamster (armenian) IgG