CST, 3329T, MNDA (3C1) Rat Monoclonal Antibody

Monoclonal Antibody for studying MNDA. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MNDA (3C1) Rat Monoclonal Antibody (CST #3329) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity MNDA (3C1) Rat Monoclonal Antibody detects endogenous levels of total MNDA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with nuclear extract from human granulocytes. Background MNDA (myeloid cell nuclear differentiation antigen) is a transcription factor constitutively expressed in peripheral blood granulocytes and monocytes; low expression is also found in a subset of B cells (1,2). MNDA is a member of the interferon (IFN)-regulated 200 family of genes, which contain one or more copies of a partially conserved domain of 200 amino acid residues thought to mediate protein-protein interaction (3). MNDA may play a role in apoptosis and its expression is reduced in myelodysplastic syndromes (MDS) (4). MNDA has been proposed to be a marker for nodal marginal zone lymphoma (5). Alternate Names epididymis secretory sperm binding protein; MNDA; Myeloid cell nuclear differentiation antigen; PYHIN3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rat IgG1