CST, 3330S, PTPA/PPP2R4 Antibody

Polyclonal Antibody for studying PPP2R4. Validated for Western Blotting. Highly specific and rigorously validated in-house, PTPA/PPP2R4 Antibody (CST #3330) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PTPA/PPP2R4 Antibody detects endogenous levels of total PTPA protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of of human PTPA. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). Active protein phosphatase 2A is composed of both structural (A) and catalytic (C) proteins, and its activity relies on interaction with regulatory (B) subunits. An important PP2A regulatory subunit is PP2A phosphatase activator (PTPA), also known as the PP2A activator regulatory subunit 4 (PPP2R4) (4). This PTPA regulatory protein binds ATP and has isomerase (PPIase) activity, suggesting that PP2A regulation involves a change in phosphatase conformation. The addition of ATP (and Mg ) results in a correlated increase in both PP2A activation and PTPA isomerase activity (5). While the exact mechanism is still under consideration, evidence suggests that binding of PTPA to the PP2A A-C dimer produces a conformational change in PP2A that shifts phosphatase substrate specificity from phosphoserine to phosphotyrosine substrates (6). Alternate Names MGC2184; Phosphotyrosyl phosphatase activator; PP2A; PP2A phosphatase activator; PP2A subunit B' isoform PR53; PP2A, subunit B'; PP2A, subunit B', PR53 isoform; PPP2R4; PR53; protein phosphatase 2 phosphatase activator; protein phosphatase 2 regulatory subunit 4; protein phosphatase 2A activator, regulatory subunit 4; protein phosphatase 2A regulatory subunit 4; protein phosphatase 2A, regulatory subunit B' (PR 53); PTPA; Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B' Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit