CST, 3332S, Jak1 Antibody

Polyclonal Antibody for studying JAK1. Validated for Western Blotting,Simple Western™. Highly specific and rigorously validated in-house, Jak1 Antibody (CST #3332) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Jak1 Antibody detects endogenous levels of total Jak1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1022/1023 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names AIIDE; JAK-1; JAK1; JAK1A; JAK1B; Janus kinase 1; Janus kinase 1 (a protein tyrosine kinase); JTK3; Tyrosine-protein kinase JAK1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 SOURCE: Rabbit