CST, 3334T, RasGRP3 (C33A3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RasGRP3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RasGRP3 (C33A3) Rabbit Monoclonal Antibody (CST #3334) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RasGRP3 (C33A3) Rabbit Monoclonal Antibody detects endogenous levels of total RasGRP3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy teminus of human RasGRP3. Background Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity (1,2). While T-Cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling (3). In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity (4,5). Alternate Names Calcium and DAG-regulated guanine nucleotide exchange factor III; calcium- and diacylglycerol-regulated guanine nucleotide exchange factor III; CalDAG-GEFIII; GRP3; Guanine nucleotide exchange factor for Rap1; KIAA0846; RAS guanyl releasing protein 3; RAS guanyl releasing protein 3 (calcium and DAG-regulated); Ras guanyl-releasing protein 3; RASGRP3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG