CST, 3336S, S5a/PSMD4 (D17E4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSMD4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, S5a/PSMD4 (D17E4) Rabbit Monoclonal Antibody (CST #3336) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity S5a/PSMD4 (D17E4) Rabbit Monoclonal Antibody recognizes endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human S5a/PSMD4 protein. Background S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5). Alternate Names 26S protease subunit S5a; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; AF-1; angiocidin; Antisecretory factor 1; ASF; MCB1; multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; proteasome 26S non-ATPase subunit 4; proteasome 26S subunit, non-ATPase 4; PSMD4; pUB-R5; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG