CST, 33386S, ETS-2 (F7A6B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ets-2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ETS-2 (F7A6B) Rabbit Monoclonal Antibody (CST #33386) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:250 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity ETS-2 (F7A6B) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETS-2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ETS-2 protein. Background ETS-2 is a member of the E26 Transformation-specific Sequence (ETS) transcription factor family, which binds to a core GGAA/T DNA sequence in the promoter region of target genes to activate gene expression (1). ETS-2 activates expression of a wide variety of target genes, including s, , , , and oncogenic miRNAs (2-4). Its activity is important for cell growth and differentiation, bone formation, and autoimmune disease and cancer progression (5-7). Phosphorylation by MAP kinase at Thr72 upregulates the function of ETS-2, suggesting that therapeutic strategies targeting ETS-2 may provide an alternative to MEK inhibitor therapy (8). Alternate Names ETS proto-oncogene 2, transcription factor; Ets-2; ETS2; ETS2IT1; oncogene ETS-2; Protein C-ets-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 61 Source/Isotype: Rabbit IgG