CST, 33388SF, IGF-II Receptor/CI-M6PR (D3V8C) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IGF2R. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, IGF-II Receptor/CI-M6PR (D3V8C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #33388) is ready to ship. Product Usage Information This product is the carrier free version of product #14364. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14364 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IGF-II Receptor/CI-M6PR (D3V8C) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total IGF-II Receptor/CI-M6PR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1675 of human IGF-II Receptor/CI-M6PR protein. Background Insulin-like growth factor II (IGF-II) receptor, also widely known as cation-independent mannose 6-phosphate receptor (CI-M6PR), is a multifunctional type I transmembrane glycoprotein that participates in the internalization of mannose-6-phosphate modified hydrolases and IGF-II from the plasma membrane (1,2). In the absence of ligands, IGF-II receptor is constitutively endocytosed from the cell surface to accumulate in the Golgi apparatus (3). In the presence of ligands, the receptor transports the mannose-6-phosphate modified hydrolases to acidified endosomes and lysosomes (4). The ligand-free receptor is then transported back to the Golgi compartment or the cell surface (4). In several research studies, IGF-II receptor has been recognized as a tumor suppressor in a number of cancers (5-7). Alternate Names 300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CI Man-6-P receptor; CI-M6PR; CI-MPR; CIMPR; IGF-II receptor; IGF2R; insulin like growth factor 2 receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P-R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300; MPR1; MPR300; MPRI Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 275 Source/Isotype: Rabbit IgG