CST, 33431SF, beta-Synuclein (F8O8P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying synuclein-beta. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, beta-Synuclein (F8O8P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #33431) is ready to ship. Product Usage Information This product is the carrier free version of product #82400. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 82400 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity beta-Synuclein (F8O8P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total β-synuclein protein. This antibody does not cross-react with α-synuclein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro90 of human β-synuclein protein. Background The synuclein family, consisting of α-synuclein, β-synuclein, and γ-synuclein, is a group of small, soluble proteins containing a highly conserved α-helical lipid-binding motif. α-synuclein and β-synuclein are primarily expressed in brain tissue, where they colocalize within presynaptic terminals (1,2). α-synuclein is linked genetically and neuropathologically to Parkinson's disease and other neurodegenerative diseases, called synucleinopathies, characterized by the presence of intracellular α-synuclein aggregates, the main component of Lewy bodies (3,4). β-synuclein has been shown to inhibit α-synuclein aggregation and ameliorate α-synuclein neurotoxicity (5-7). Studies have shown that β-synuclein levels are elevated in early and advanced Alzheimer's disease, Creutzfeldt-Jakob disease, and traumatic brain injury, showing strong potential for use as a synaptic damage biomarker (8-9). Alternate Names Beta-synuclein; I(2)-synuclein; SNCB; synuclein beta; synuclein, beta; SYUB Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG