CST, 33443S, SHMT2 (E7F4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHMT2. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SHMT2 (E7F4Q) Rabbit Monoclonal Antibody (CST #33443) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity SHMT2 (E7F4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHMT2 protein. This antibody does not cross-react with SHMT1 protein. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly451 of human SHMT2 protein. Background Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4). Alternate Names epididymis secretory sperm binding protein Li 51e; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; GLYM; HEL-S-51e; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2; serine hydroxymethyltransferase 2 (mitochondrial); Serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; SHMT2; threonine aldolase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG