CST, 3344T, Jak1 (6G4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JAK1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Jak1 (6G4) Rabbit Monoclonal Antibody (CST #3344) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77692 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Jak1 (6G4) Rabbit Monoclonal Antibody detects endogenous levels of total Jak1 protein. This antibody does not cross react with other Jak family members. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile800 of Jak1. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names AIIDE; JAK-1; JAK1; JAK1A; JAK1B; Janus kinase 1; Janus kinase 1 (a protein tyrosine kinase); JTK3; Tyrosine-protein kinase JAK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG