CST, 33469S, IgG (RWP49) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IgG1 heavy chain constant. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, IgG (RWP49) Mouse Monoclonal Antibody (CST #33469) is ready to ship. Product Usage Information IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied as liquid tissue culture supernatant containing 0.35% ProClin™ 950 as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity IgG (RWP49) Mouse Monoclonal Antibody recognizes endogenous levels of IgG protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 327 amino acids of human IgG protein. Background Immunoglobulin G (IgG) is one of the five major human immunoglobulin isotypes and consists of two identical heavy chains and two identical light chains, linked by disulfide bonds (1). There are four subclasses of IgG antibodies: IgG1, IgG2, IgG3, and IgG4. The disulfide bond structure of each subclass is slightly different and can impact antibody stability and function (2). IgG is the most abundant immunoglobulin isotype found in human serum, helping to protect the body from viral, bacterial, and fungal pathogens (3). IgG antibodies are expressed by B cells and functionally contribute to both the innate and adaptive arms of the immune response (4). The presence of IgG antibodies in the respiratory tract and mucosal layers make it important when studying infections, such as respiratory syncytial virus (RSV) and SARS-CoV-2 (5,6). Alternate Names constant region of heavy chain of IgG1; Ig gamma-1 chain C region; Ig gamma-1 chain C region EU; Ig gamma-1 chain C region KOL; Ig gamma-1 chain C region NIE; IGHG1; immunoglobulin gamma 1 (Gm marker); Immunoglobulin heavy constant gamma 1; immunoglobulin heavy constant gamma 1 (G1m marker) Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1