CST, 33483S, BOB-1/OBF-1 Antibody

Polyclonal Antibody for studying POU2AF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, BOB-1/OBF-1 Antibody (CST #33483) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BOB-1/OBF-1 Antibody recognizes endogenous levels of total BOB-1/OBF-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human BOB-1/OBF-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background B cell Oct binding factor-1 (BOB-1/OBF-1) is a B cell restricted transcriptional coactivator. BOB-1 facilitates transactivation of immunoglobulins and other B cell specific genes through the binding and activation of the transcription factors Oct-1 and Oct-2 (1-4). Research studies have demonstrated that BOB-1 expression is required for antigen-dependent B cell maturation (5-7). In pathological conditions such as classical Hodgkin disease, loss of BOB-1 expression is thought, in part, to contribute to the defect in immunoglobulin gene expression by Hodgkin and Reed Sternberg cells (8,9). In the context of multiple myeloma, overexpression of BOB-1 has been shown to contribute to malignant plasma cell growth, in part, through enhanced transactivation of (10). Alternate Names B-cell-specific coactivator OBF-1; BOB-1; BOB1; OBF-1; OBF1; OCA-B; OCAB; OCT-binding factor 1; POU class 2 associating factor 1; POU class 2 homeobox associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1; POU2AF1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit