CST, 33484SF, IL-18 (E3G8R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IL18. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-18 (E3G8R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #33484) is ready to ship. Product Usage Information This product is the carrier free version of product #67775. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 67775 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IL-18 (E3G8R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IL-18 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human IL-18 recombinant protein. Background Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7). Alternate Names Iboctadekin; IFN-gamma-inducing factor; IGIF; IL 18; IL-1 gamma; IL-18; IL-1g; IL18; IL1F4; Interferon gamma-inducing factor; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; MGC12320 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG