CST, 3349S, SGTA Antibody

Polyclonal Antibody for studying SGTA. Validated for Western Blotting. Highly specific and rigorously validated in-house, SGTA Antibody (CST #3349) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SGTA Antibody detects endogenous levels of total SGTA protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SGTA. Antibodies are purified by protein A and peptide affinity chromatography. Background SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6). Alternate Names Alpha-SGT; alphaSGT; hSGT; protein containing three tetratricopeptide repeats; SGT; SGT1; SGTA; small glutamine rich tetratricopeptide repeat containing alpha; small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; UBP; Vpu-binding protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 34 SOURCE: Rabbit