CST, 33510S, UTX (D3Q1I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UTX. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, UTX (D3Q1I) Rabbit Monoclonal Antibody (CST #33510) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity UTX (D3Q1I) Rabbit Monoclonal Antibody recognizes endogenous levels of total UTX protein. This antibody does not cross-react with UTY protein. This antibody also cross-reacts with unidentified proteins of 60 kDa and 70 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Ala490 of human UTX protein. Background The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The three members of the UTX/UTY family include the ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX), the ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY), and JmjC domain-containing protein 3 (JMJD3) (3). This family of proteins has been shown to demethylate both di- and tri-methyl histone H3 Lys 27 (4-8). The gene escapes X inactivation in females and is ubiquitously expressed (9). UTX functions to regulate gene expression during development (4-6). JMJD3 functions to regulate gene expression in macrophages responding to various inflammatory stimuli and has been shown to be upregulated in prostate cancer (7,8). Both UTX and JMJD3 interact with mixed-lineage leukemia (MLL) complexes 2 and 3, both of which have been shown to methylate histone H3 at Lys4 (6,7). The gene is expressed in most tissues in the male mouse (10). Alternate Names [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); DKFZp686A03225; Histone demethylase UTX; KABUK2; KDM6A; lysine (K)-specific demethylase 6A; lysine demethylase 6A; Lysine-specific demethylase 6A; MGC141941; ubiquitously transcribed tetratricopeptide repeat protein X-linked; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein; UTX Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG