CST, 3354S, CACYBP Antibody

Polyclonal Antibody for studying CACYBP. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CACYBP Antibody (CST #3354) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CACYBP Antibody detects endogenous levels of total CACYBP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp162 of human CACYBP. Antibodies are purified by peptide affinity chromatography. Background Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9). Alternate Names CACYBP; calcyclin binding protein; Calcyclin-binding protein; CYBP; GIG5; growth-inhibiting gene 5 protein; hCacyBP; MGC87971; PNAS-107; RP1-102G20.6; S100A6-binding protein; S100A6BP; Siah-interacting protein; Siah-interacting protein (SIP); SIP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 26 SOURCE: Rabbit