CST, 3357S, CaMKII-alpha Antibody

Polyclonal Antibody for studying CaMK2-alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, CaMKII-alpha Antibody (CST #3357) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CaMKII-alpha Antibody detects endogenous levels of total CaMKII-α. The peptide sequence used as the antigen is not conserved in CaMKII-β, -γ and -δ. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr436 of human CaMKII-α. . Antibodies are purified by protein A and peptide affinity chromatography. Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca /calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4). Alternate Names calcium/calmodulin dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; calmodulin-dependent kinase II; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMK-II subunit alpha; CAMK2A; CAMKA; CaMKII; CaMKIIalpha; CaMKIINalpha; KCC2A; KIAA0968; MRD53; MRT63 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit