CST, 3360S, RagC Antibody

Polyclonal Antibody for studying RagC. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, RagC Antibody (CST #3360) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RagC Antibody detects endogenous levels of total RagC protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human RagC protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). Alternate Names FLJ13311; GTPase-interacting protein 2; GTR2; Rag C; Rag C protein; RagC; Ras related GTP binding C; Ras-related GTP binding C; Ras-related GTP-binding protein C; RRAGC; TIB929 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit