CST, 3363T, Dicer Antibody

Polyclonal Antibody for studying Dicer1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Dicer Antibody (CST #3363) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dicer Antibody detects endogenous levels of total Dicer protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1136 of human Dicer protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3). Alternate Names DCR1; DICER; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease III; dicer 1, ribonuclease type III; DICER1; Dicer1, Dcr-1 homolog; Dicer1e; Endoribonuclease Dicer; GLOW; Helicase MOI; Helicase with RNase motif; helicase-moi; HERNA; K12H4.8-LIKE; KIAA0928; MNG1; RMSE2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 SOURCE: Rabbit