CST, 3366S, Neuropilin-2 (D39A5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Neuropilin2 mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Neuropilin-2 (D39A5) Rabbit Monoclonal Antibody (CST #3366) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:500 - 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Neuropilin-2 (D39A5) Rabbit Monoclonal Antibody detects endogenous levels of total neuropilin-2 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Neuropilin-2 protein. Background Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single-pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Neuropilin-2, a single-pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g. pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5). Alternate Names 1110048P06Rik; neuropilin 2; Neuropilin-2; Np-2; Np2; Npn-2; Npn2; Nrp2; OTTMUSP00000002407; OTTMUSP00000002408; OTTMUSP00000002409; OTTMUSP00000002410; OTTMUSP00000002411; OTTMUSP00000002412; RP23-149A5.1; Vascular endothelial cell growth factor 165 receptor 2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG