CST, 33699SF, HES1 (D6P2U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HES1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, HES1 (D6P2U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #33699) is ready to ship. Product Usage Information This product is the carrier free version of product #11988. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 11988 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HES1 (D6P2U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HES1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human HES1 protein. The epitope has been mapped to residues surrounding Ala230. Background HES1 (Hairy and Enhancer of Split 1) is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors which function primarily to repress transcription of bHLH-dependent genes (1). HES1 is understood to play an important conserved role in maintaining pluripotency of embryonic and adult stem/progenitor cells via the transcriptional repression of genes that promote differentiation (1,2). HES1 is particularly well known as a repressive mediator of the canonical Notch signaling pathway (3). HES1 plays a key role in mediating Notch-dependent T cell lineage commitment (4), and has been reported to be an essential mediator of Notch-induced T cell acute lymphoblastic leukemia (T-ALL) (4,5). HES1 is also reported to mediate Notch-induced repression of differentiation in a number of cancer cell types. A conditional deletion of HES1 from intestinal tumor cells in APC-mutant mice reduced tumor cell proliferation, while promoting differentiation toward epithelial lineages (6). Overexpression of HES1 in a human osteosarcoma (OS) cell line was shown to repress expression of the Notch antagonist , leading to increased OS cell invasiveness (7). Other genes subject to transcriptional repression by HES1 include , and the NOTCH ligands and (6,8,9). Alternate Names BHLHB39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; hes family bHLH transcription factor 1; HES-1; HES1; hHL; HL; HRY; Transcription factor HES-1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG