CST, 3372S, Erk5 Antibody

Polyclonal Antibody for studying ERK5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Erk5 Antibody (CST #3372) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Erk5 Antibody detects endogenous levels of total Erk5 protein. This antibody does not cross-react with Erk1 or Erk2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala497 of human Erk5. Antibodies are purified by protein A and peptide affinity chromatography. Background Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2). Alternate Names Big MAP kinase 1; BMK-1; BMK1; BMK1 kinase; ERK-5; ERK4; ERK5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen-activated protein kinase 7; MK07; PRKM7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 115 SOURCE: Rabbit