CST, 33839SF, WHSC1L1 (D4N9N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying WHSC1L1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, WHSC1L1 (D4N9N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #33839) is ready to ship. Product Usage Information This product is the carrier free version of product #92056. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 92056 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity WHSC1L1 (D4N9N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total WHSC1L1 protein, both long and short isoforms. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro117 of human WHSC1L1 protein. Background Wolf-Hirschhorn syndrome candidate 1-like protein 1 (WHSC1L1), also known as histone-lysine N-methyltransferase NSD3, is a SET domain-containing histone methyltransferase protein that methylates histone H3 on lysine 4 (H3K4me) and lysine 27 (H3K27me) (1). Methylation of histone H3 lysine 4 is associated with transcriptional activation, while methylation of histone H3 lysine 27 is associated with transcriptional repression. WHSC1L1 can function as an oncogene or a tumor suppressor protein, depending on cell context, and has been shown to regulate expression of a number of genes associated with cell cycle (2). Amplification and/or increased expression of WHSC1L1 in breast, lung, and liver cancer increases growth and survival, and is associated with poor prognosis (2-5). In addition, the NSD3 gene has been found fused with the nuclear pore complex protein NUP98 gene in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), and fused with the NUT gene in NUT midline carcinomas (6-10). Alternate Names DKFZp667H044; FLJ20353; Histone-lysine N-methyltransferase NSD3; KMT3F; KMT3G; MGC126766; MGC142029; NSD3; nuclear receptor binding SET domain protein 3; Nuclear SET domain-containing protein 3; pp14328; Protein whistle; WHISTLE; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; WHSC1L1; Wolf-Hirschhorn syndrome candidate 1-like 1; Wolf-Hirschhorn syndrome candidate 1-like 1 protein; Wolf-Hirschhorn syndrome candidate 1-like protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80-90, 180 Source/Isotype: Rabbit IgG