CST, 3389S, LPP (8B3A11) Mouse Monoclonal Antibody

Monoclonal Antibody for studying LPP. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, LPP (8B3A11) Mouse Monoclonal Antibody (CST #3389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LPP (8B3A11) Mouse Monoclonal Antibody detects endogenous levels of total LPP protein. Species Reactivity: Human, Mouse, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP. Background LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1). LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3). Alternate Names DKFZp779O0231; FLJ30652; FLJ41512; LIM domain containing preferred translocation partner in lipoma; LIM domain-containing preferred translocation partner in lipoma; LIM protein; lipoma preferred partner; Lipoma-preferred partner; LPP Specification REACTIVITY: H M Hm Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Mouse IgG1