CST, 33942S, SWAP70 (D5O6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SWAP70. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SWAP70 (D5O6A) Rabbit Monoclonal Antibody (CST #33942) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SWAP70 (D5O6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SWAP70 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human SWAP70 protein. Background SWAP70 is a Rac family guanine nucleotide exchange factor (GEF) (1). It is highly expressed by activated B cells. Following B cell stimulation, SWAP70 has been observed to translocate from the cytoplasm to the nucleus, where it plays a role in class switching, as well as to the membrane, where it associates with the B cell receptor (2,3). SWAP70 also plays a role in migration of B cells and other immune cell types including dendritic cells, eosinophils, and mast cells (4-7). Mice deficient in both SWAP70 and a related protein, DEF6, develop lupus-like autoimmunity due to misregulation of IRF4 in B cells and T cells leading to increased IL21 production and responsiveness (8). Alternate Names FLJ39540; HSPC321; KIAA0640; SWAP switching B-cell complex 70kDa subunit; SWAP switching B-cell complex subunit 70; SWAP-70; SWAP70; Switch-associated protein 70; switching B cell complex subunit SWAP70; SWP70 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70, 74 Source/Isotype: Rabbit IgG